Cross-reacting human and rabbit antibodies to antigens of Histoplasma capsulatum, Candida albicans, and Saccharomyces cerevisiae.

Using Western blots of electrophoretically separated antigens, we show that human antibodies react most frequently to antigens shared by three fungi (Histoplasma capsulatum, Candida albicans, and Saccharomyces cerevisiae). Reactivity to antigens specific for individual fungi was relatively uncommon. The pattern of reactivity could not distinguish infected patients from uninfected controls. Rabbits immunized with extracts of each fungus also produced antibodies to cross-reactive or shared antigens of the other two fungi. Furthermore, preimmune sera showed similar but lower reactivity with the same fungal antigens. We believe that the preimmunization antibodies, which probably resulted from earlier fungal colonization or inapparent infections, predisposed the immune responses elicited by the vaccinations. A similar mechanism likely explains the results with human sera.